| Id |
Target |
Expression Suffix |
Clone |
Local Clone |
Local Protein Target |
Local Expression |
Experiment Date Start |
Experiment Date End |
Staff |
Lab |
Protocol |
Status |
Expression Organism |
Expression Strain |
Media Type |
Media Volume |
Growth Temperature |
Induction Temperature |
Induction Reagent |
Experiment Type |
Expression Level |
Solubility Level |
Actions |
|
2835 |
IDP01174 |
2 |
2502 |
|
|
J0002472 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
10 |
0 |
View Edit Delete |
|
6064 |
IDP01162 |
3 |
3083 |
|
|
|
Oct 13, 2008 |
Oct 14, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9132 |
IDP04403 |
1 |
6360 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
2818 |
IDP01162 |
2 |
2485 |
|
|
J0002448 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
5 |
0 |
View Edit Delete |
|
2693 |
IDP01155 |
2 |
2360 |
|
|
J0002434 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3299 |
IDP01156 |
1 |
2966 |
|
|
J0002435 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9063 |
IDP04287 |
1 |
6291 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
6086 |
IDP01174 |
3 |
3100 |
|
|
|
Oct 13, 2008 |
Oct 14, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9133 |
IDP04404 |
1 |
6361 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
1566 |
IDP00038 |
3 |
1679 |
|
|
J0001329 |
Apr 25, 2008 |
Apr 26, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-pLysS |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
2539 |
IDP01088 |
2 |
2206 |
|
|
J0002314 |
Sep 16, 2008 |
Sep 17, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3391 |
IDP01140 |
1 |
3058 |
|
|
J0002401 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
13674 |
IDP01014 |
60104 |
9257 |
|
|
|
Aug 29, 2011 |
|
Veronica Yim |
UT |
Expression_protocol_Toronto |
failed |
E. coli |
BL21-CodonPlus(DE3)-RIL |
Studier w/o lactose |
0.00075 |
37 |
18 |
none |
production |
0 |
0 |
View Edit Delete |
|
6159 |
IDP01231 |
4 |
2465 |
|
|
|
Oct 14, 2008 |
Oct 15, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3390 |
IDP01229 |
1 |
3057 |
|
|
J0002581 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9122 |
IDP04386 |
1 |
6350 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
9123 |
IDP04387 |
1 |
6351 |
|
|
|
Nov 30, 2009 |
Dec 07, 2009 |
Sarah Grimshaw |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
success |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
30 |
0 |
View Edit Delete |
|
6158 |
IDP01231 |
3 |
3063 |
|
|
|
Oct 13, 2008 |
Oct 14, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
3433 |
IDP01174 |
1 |
3100 |
|
|
J0002471 |
Sep 15, 2008 |
Sep 16, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
M9 minimal |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |
|
6101 |
IDP01183 |
4 |
2356 |
|
|
|
Oct 14, 2008 |
Oct 15, 2008 |
Jason Stam |
JCVI |
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins |
failed |
E. coli |
BL21(DE3)-Magic |
2xYT |
0.15 |
37 |
25 |
1mM IPTG |
assay |
0 |
0 |
View Edit Delete |