Clone

Target

IDP90564  

Clone Suffix

1  

Sequence Comments

 

Local Protein Target ID

 

Local Clone Plate ID

 

Local Clone ID

4827  

Experiment Start Date

2009-10-28  

Experiment End Date

2009-10-28  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

632  

Reference Sequence Used

MNIQILQEQA NTLRFLSADM VQKANSGHPG APLGLADILS VLSYHLKHNP KNPTWLNRDR LVFSGGHASA LLYSFLHLSG YDLSLEDLKN FRQLHSKTPG HPEISTLGVE IATGPLGQGV ANAVGFAMAA KKAQNLLGSD LIDHKIYCLC GDGDLQEGIS YEACSLAGLH KLDNFILIYD SNNISIEGDV GLAFNENVKM RFEAQGFEVL SINGHDYEEI NKALEQAKKS TKPCLIIAKT TIAKGAGELE GSHKSHGAPL GEEVIKKAKE QAGFDPNISF HIPQASKIRF ESAVELGDLE EAKWKDKLEK SAKKELLERL LNPDFNKIAY PDFKGKDLAT RDSNGEILNV LAKNLEGFLG GSADLGPSNK TELHSMGDFV EGKNIHFGIR EHAMAAINNA FARYGIFLPF SATFFIFSEY LKPAARIAAL MKIKHFFIFT HDSIGVGEDG PTHQPIEQLS TFRAMPNFLT FRPADGVENV KAWQIALNAD IPSAFVLSRQ KLKALNEPVF GDVKNGAYLL KESKEAKFTL LASGSEVWLC LESANELEKQ GFACNVVSMP CFELFEKQDK AYQERLLKGE VIGVEAAHSN ELYKFCHKVY GIESFGESGK DKDVFERFGF SVSKLVNFIL SK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MNIQILQEQA NTLRFLSADM VQKANSGHPG APLGLADILS VLSYHLKHNP KNPTWLNRDR LVFSGGHASA LLYSFLHLSG YDLSLEDLKN FRQLHSKTPG HPEISTLGVE IATGPLGQGV ANAVGFAMAA KKAQNLLGSD LIDHKIYCLC GDGDLQEGIS YEACSLAGLH KLDNFILIYD SNNISIEGDV GLAFNENVKM RFEAQGFEVL SINGHDYEEI NKALEQAKKS TKPCLIIAKT TIAKGAGELE GSHKSHGAPL GEEVIKKAKE QAGFDPNISF HIPQASKIRF ESAVELGDLE EAKWKDKLEK SAKKELLERL LNPDFNKIAY PDFKGKDLAT RDSNGEILNV LAKNLEGFLG GSADLGPSNK TELHSMGDFV EGKNIHFGIR EHAMAAINNA FARYGIFLPF SATFFIFSEY LKPAARIAAL MKIKHFFIFT HDSIGVGEDG PTHQPIEQLS TFRAMPNFLT FRPADGVENV KAWQIALNAD IPSAFVLSRQ KLKALNEPVF GDVKNGAYLL KESKEAKFTL LASGSEVWLC LESANELEKQ GFACNVVSMP CFELFEKQDK AYQERLLKGE VIGVEAAHSN ELYKFCHKVY GIESFGESGK DKDVFERFGF SVSKLVNFIL SK  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAACATTCAAATTTTACAAGAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTGCTAAGGATAAAATTTACA  

Shipped To Lab ID

ANL  

Shipped Date

2009-11-03