Center for Structural Genomics of Infectious Diseases - Purifications

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Csgid ID: IDP00487

Page 1 of 1, showing 7 records out of 7 total, starting on record 1, ending on 7

Id	Target	Purification Suffix	Expression	Local Expression	Local Protein Target	Local Purification	Experiment Date Start	Experiment Date End	Staff	Lab	Protocol	Status	Description	Affinity Tag Status	Semet Status	Covalent Modification	Final Buffer	Final Concentration	Final Volume	Shipped To Lab	Shipped Date	Actions
7697	IDP00487	3120	12979	1135	5IfUCEIoCmy4zNUQEAqVoFyoQ7w	3120	Oct 21, 2008	Oct 23, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	na	semet		50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-10-23	View Edit Delete
7696	IDP00487	3121	12979	1135	5IfUCEIoCmy4zNUQEAqVoFyoQ7w	3121	Oct 21, 2008	Oct 23, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	na	semet		50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-10-23	View Edit Delete
7695	IDP00487	3122	12979	1135	5IfUCEIoCmy4zNUQEAqVoFyoQ7w	3122	Oct 21, 2008	Oct 23, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	na	semet		50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-10-23	View Edit Delete
7694	IDP00487	4234	12979	1135	5IfUCEIoCmy4zNUQEAqVoFyoQ7w	4234	Oct 21, 2008	Oct 23, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	na	semet		50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-10-23	View Edit Delete
7693	IDP00487	4235	12979	1135	5IfUCEIoCmy4zNUQEAqVoFyoQ7w	4235	Oct 21, 2008	Oct 23, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::SEC::SEC peak 1	na	semet		50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-10-23	View Edit Delete
7692	IDP00487	4236	12979	1135	5IfUCEIoCmy4zNUQEAqVoFyoQ7w	4236	Oct 21, 2008	Oct 23, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	na	semet		50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-10-23	View Edit Delete
7691	IDP00487	4237	12979	1135	5IfUCEIoCmy4zNUQEAqVoFyoQ7w	4237	Oct 21, 2008	Oct 23, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	na	semet		50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-10-23	View Edit Delete

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