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Target Request


Target Identification:

(If the proposed target is included in the NCBI protein database, please enter its gi # and click "load data from NCBI", or click "selection db lookup" to search in target selection database )

Input Protein GI Number*

Target Properties and Relevance of Selection:

Protein Description
Defline
NCBI Acc. Code
Protein Sequence*
DNA Sequence*

Organism & Strain:

CSGID can clone the requested gene from any strain that has been sequenced completely and its genomic DNA is available ( http://atcc.org/CulturesandProducts/Microbiology/DNAandRNA/tabid/181/Default.aspx#bacteria ). However, if provide your own clone/genomic DNA or choose one of the available in-house strains ( http://www.jcvi.org/cms/research/projects/csgid/available-organisms/ ), your request may be able to proceed faster.

Organism
Source of
genomic DNA*  
Strain*

Justification* (A brief (one or two sentences) description of how determination of target structure will enhance research progress in the field. The justification may include reference to supporting experimental evidence and/or relevant literature, as well as the approximate size of the relevant scientific community.)

Comments (special information relevant to the proposed approach for structure determination (e.g. the importance of post-translational protein modifications, known ligands and their availability, previous attempts at either X-ray crystallography or NMR structure determination, and whether one of the centers is specifically requested). This information is optional, but may be of significant value in determining the appropriate protein production and structure determination pipelines)

Resources* (describe the availability of genetic material suitable for expression of the target protein, including any molecular clones already available)


Applicant's Information:

First Name*
Last Name*
Institution*
Email*

Request is not complete until you click "Submit". Following submission, you should be receiving confirmation email from CSGID.

Submission:
By clicking "Submit" I agree that structures determined by the CSGID will be submitted to the PDB and be part of the public domain.

Contact Us:
Proposals which do not fit this form, nonstandard requests etc. may be submitted directly by e-mail. Any correspondence regarding your proposal should be also sent to this address.

 
page last updated on October 14th 2009
Fields marked by '*' are obligatory