Structure of IDP90246

Crystal structure of the catalytic domain of DapE protein from V.cholerea in the Zn bound form

(tree view)
Edit deposit information
CSGID target
IDP90246 
PDB Id
4OP4 (NCBI MMDB) 
Authors
'B.Nocek,M.Makowska-Grzyska,R.Jedrzejczak,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid)' 
Responsible person
Boguslaw Nocek 
Responsible lab
Argonne National Laboratory 
Deposition Date
Feb 04, 2014 
Release Date
Apr 23, 2014 

Annotation

Edit this annotation
Description
DapE is a member of the meso-diaminopimelate (mDAP)/lysine biosynthetic pathway. The amino acids mDAP and/or lysine are essential components of the peptidoglycan cell wall for Gram-negative and most Gram-positive bacteria, providing a link between polysaccharide strands. Therefore, enzymes involved in the mDAP/lysine biosynthetic pathway are all potential antibiotic targets. DapEs are dimeric metallo-enzymes (41.6 kDa/subunit) that require two atoms of zinc per mole of polypeptide for full enzymatic activity.  
Functional assignment
DapE enzyme 

Ligands

Ligand code Name Ligand type
BU1 crystallization
Zn biological
GOL glycerol crystallization
175 3,5-dihydro-5-methylidene-4h-imidazol-4-on

Structure information

View structure information | Edit structure information (upload a PDB file)

Unit cell parameters

Space Group
P 32 2 1  
Unit Cell

a=49.86Å, b=49.86Å, c=231.76Å
α=90.00, β=90.00, γ=120.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
40.00-1.65Å (1.69-1.65Å)  
Rall(%)
15.5 
Rwork(%)
15.4 (27.3) 
Rfree(%)
18.2 (33.9) 
Num. observed reflections
37179 (1662) 
Num. Rfree reflections
1858 (81) 
Completeness(%)
89.3 (55.0) 

Model parameters

Num Atoms
2286  
Num Waters
243  
Num Hetatoms
2  
Model mean isotropic B factor
20.070Å2  
RMSD bond length
0.016Å  
RMSD bond angle
1.501°  
Filename uploaded
DapEVCZnZNEDO-DEPOSIT-0728-2-correct.pdb (uploaded on Jul 30, 2011 4:12 PM)  
Inserted
Jul 30, 2011