Structure of IDP91183

Structure of an autocleavage-inactive mutant of the cytoplasmic domain of CT091, the YscU homologue of Chlamydia trachomatis

Edit deposit information
CSGID target
IDP91183 
PDB Id
3T7Y (NCBI MMDB
Authors
A.U.Singer,Z.Wawrzak,T.Skarina,P.Saikali,W.F.Anderson,A.Savchenko,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Alexander Singer 
Responsible lab
University of Calgary 
Deposition Date
Jul 31, 2011 
Release Date
Nov 16, 2011 

Annotation

Description
The YscU family of proteins are one of several proteins involved in assembly of the type III apparatus in gram-negative pathogenic and symbiotic bacteria. This protein in particular is important in the maturing of the type III pilus as it switches from secreting pilus subunits to secreting type III effectors. YscU-type proteins contain a transmembrane region within the N-terminal ~200 residues followed by a cytoplasmic domain; this cytoplasmic domain undergoes an auto-cleavage at a highly conserved Asn residue and this cleavage is key to the maturing of the type III pilus. Structures of the cytoplasmic domain of YscU from Yersinia, E. coli and Shigella have been solved in their non-mutated (cleaved) form (for example see PDB 2JLI and 2VT1) and mutated forms which are cleavage-inactive (PDB 2JLH, 2JLJ, 2W0R and 3C00) to determine differences between the pre- and post-cleaved conformation relevant for pilus maturation. As part of our understanding of the pilus maturation process we have undertaken structure determination of a number of proteins involved in this process including the chlamydial homologue of YscO (CT670, PDB 3K29) and the cleavage inactive cytoplasmic domain of the chlamydial YscU described here. Despite the ~30% sequence homology with YscU homologues whose structures have been reported, we find the chlamydial structure is virtually identical to those of other reported YscU homologues. 
Functional assignment
type III apparatus 

Ligands

Ligand code Name Ligand type
FMT crystallization
DMS dimethyl sulfoxide crystallization
NA Sodium ion crystallization
CL CHLORIDE ION crystallization
175 3,5-dihydro-5-methylidene-4h-imidazol-4-on

Structure information

Unit cell parameters

Space Group
P 31  
Unit Cell

a=68.36Å, b=68.36Å, c=37.34Å
α=90.00, β=90.00, γ=120.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
31.58-2.10Å (0.00-0.00Å)  
Rall(%)
18.1 
Rwork(%)
17.9 (0.0) 
Rfree(%)
21.1 (0.0) 
Num. observed reflections
11833 (0) 
Num. Rfree reflections
573 (0) 
Completeness(%)
99.0 (0.0) 

Model parameters

Num Atoms
1655  
Num Waters
79  
Num Hetatoms
0  
Model mean isotropic B factor
33.210Å2  
RMSD bond length
0.000Å  
RMSD bond angle
0.000°  
Filename uploaded
ct91mut_refine_32.pdb (uploaded on Aug 05, 2011 12:11 PM)  
Inserted
Aug 05, 2011