Structure of IDP90515

30S ribosomal protein S15 from Campylobacter jejuni

Edit deposit information
CSGID target
IDP90515 
PDB Id
4IYL (NCBI MMDB
Authors
J.Osipiuk,B.Nocek,M.Gu,K.Kwon,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Jerzy Osipiuk 
Responsible lab
Argonne National Laboratory 
Deposition Date
Jan 28, 2013 
Release Date
Nov 30, 2011 

Annotation

Description
The 30S, small subunit of the bacterial ribosome is comprised of one large ribosomal RNA (rRNA), 16S rRNA, and about 21 unique ribosomal proteins (r-proteins: S1–S21). S15 is a primary binding protein that plays a pivotal role in the assembly of ribosomal proteins S6, S11, S18, and S21 with the central domain of 16S ribosomal RNA to form the platform of the 30S subunit. 30S subunits lacking S15 appear to be somewhat defective in subunit association in vivo and in vitro. S15 regulates its own expression by a feedback mechanism at the translational level. The presented structure of C. jejuni S15 shows unique ‘unfolded’ conformation compared to previously known S15 structures. 
Functional assignment
ribosomal protein 

Ligands

Ligand code Name Ligand type

Structure information

Unit cell parameters

Space Group
I 2 2 2  
Unit Cell

a=43.24Å, b=58.63Å, c=115.87Å
α=90.00, β=90.00, γ=90.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
32.27-2.36Å (2.42-2.36Å)  
Rall(%)
24.7 
Rwork(%)
24.6 (35.8) 
Rfree(%)
28.1 (37.3) 
Num. observed reflections
5992 (366) 
Num. Rfree reflections
275 (15) 
Completeness(%)
94.3 (79.4) 

Model parameters

Num Atoms
678  
Num Waters
4  
Num Hetatoms
4  
Model mean isotropic B factor
81.750Å2  
RMSD bond length
0.012Å  
RMSD bond angle
1.611°  
Filename uploaded
x22h_refmac4.pdb (uploaded on Jan 28, 2013 5:45 PM)  
Inserted
Nov 21, 2011