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Structure of IDP01325

High resolution structure of a putative organic hydroperoxide resistance protein from Vibrio cholerae O1 biovar eltor str. N16961

Edit deposit information
CSGID target
IDP01325 
PDB Id
3EER (NCBI MMDB
Authors
B.Nocek,N.Maltseva,K.Kwon,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Boguslaw Nocek 
Responsible lab
Argonne National Laboratory 
Deposition Date
Sep 05, 2008 
Release Date
Oct 21, 2008 

Annotation

Description
The organic hydroperoxide resistance protein, thiol-dependent peroxidase that is a central player in response to stress induced by organic hydroperoxides in bacteria. It has unique three-dimensional structure and requires dithiols to support its activity. It forms an oval-shaped, tight homodimer and have two active site positioned on the opposite faces of the dimer. We have determined series of the structure of OHR-like protein from V. cholera and were able to reveal the enzymes structure in the reduce state (3I07), in the presence of captopril molecule (3LUS). FOX assays confirmed that it metabolizes hydrogen peroxide. 
Functional assignment
OHR 

Ligands

Ligand code Name Ligand type
ACT acetate
IMD
ZN ZINC ION
MSE modified residue

Structure information

Unit cell parameters

Space Group
I 21 21 21  
Unit Cell

a=59.65Å, b=71.13Å, c=71.63Å
α=90.00, β=90.00, γ=90.00 
Solvent content
50.02  
Matthews coefficient
2.46  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
40.00-1.45Å (1.49-1.45Å)  
Rall(%)
14.5 
Rwork(%)
13.9 (21.5) 
Rfree(%)
16.4 (27.1) 
Num. observed reflections
27216 (1820) 
Num. Rfree reflections
1360 (103) 
Completeness(%)
99.0 (90.6) 

Model parameters

Num Atoms
1017  
Num Waters
182  
Num Hetatoms
236  
Model mean isotropic B factor
5.820Å2  
RMSD bond length
0.015Å  
RMSD bond angle
1.508°  
Filename uploaded
3EER.pdb (uploaded on Sep 16, 2009 5:03 PM)  
Inserted
Oct 27, 2008