Annotation

Description

Dihydroorotase (EC 3.5.2.3) catalyzes the third step in pyrimidine biosynthesis, namely, the reversible cyclization of carbamoyl aspartate to form dihydroorotate. In humans, the amidohydrolase activity of dihyroorotate (DHO), which is the reverse of the biosynthetic reaction, is responsible for inactivating the cardioprotective drug, dexrazoxane. DHO is a two-domain structure, one domain is formed by the N and C-terminus (1-55 and 366–422) and a distorted TIM barrel. 

Functional assignment

 

Structure information

Unit cell parameters

Space Group

P 1  

Unit Cell

a=50.22Å, b=55.23Å, c=85.65Å
α=88.33, β=76.56, γ=76.97 

Solvent content

48.78  

Matthews coefficient

2.4  

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

30.00-2.00Å (2.05-2.00Å)  

R_all(%)

19.1 

R_work(%)

18.9 (22.7) 

R_free(%)

23.7 (28.7) 

Num. observed reflections

57572 (4201) 

Num. R_free reflections

2936 (207) 

Completeness(%)

97.8 (96.5) 

Model parameters

Num Atoms

6952  

Num Waters

478  

Num Hetatoms

0  

Model mean isotropic B factor

26.700Ų  

RMSD bond length

0.013Å  

RMSD bond angle

1.436°  

Filename uploaded

b8_deposit_idp795.pdb (uploaded on Mar 31, 2009 12:37 PM)  

Inserted

Mar 31, 2009