Annotation

Description

YqeH is an essential GTP-binding protein. Depletion of YqeH induces an excess initiation of DNA replication, suggesting that it negatively controls initiation of chromosome replication. The YqeH subfamily is common in eukaryotes and sporadically present in bacteria with probable acquisition by plants from chloroplasts. Proteins of the YqeH family contain all sequence motifs typical of the vast class of P-loop-containing GTPases, but show a circular permutation, with a G4-G1-G3 pattern of motifs as opposed to the regular G1-G3-G4 pattern seen in most GTPases. 

Functional assignment

GTP Binding 

Structure information

Unit cell parameters

Space Group

C 1 2 1  

Unit Cell

a=83.13Å, b=58.99Å, c=77.72Å
α=90.00, β=99.38, γ=90.00 

Solvent content

45.52  

Matthews coefficient

2.26  

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

76.70-1.80Å (1.85-1.80Å)  

R_all(%)

20.9 

R_work(%)

20.8 (25.2) 

R_free(%)

23.7 (28.1) 

Num. observed reflections

34007 (2494) 

Num. R_free reflections

1700 (121) 

Completeness(%)

98.4 (97.8) 

Model parameters

Num Atoms

2586  

Num Waters

126  

Num Hetatoms

0  

Model mean isotropic B factor

52.980Ų  

RMSD bond length

0.016Å  

RMSD bond angle

1.390°  

Filename uploaded

idp90222.pdb (uploaded on Apr 15, 2009 7:55 AM)  

Inserted

Apr 15, 2009