To see full content of that page you need the activeISee plugin.
Get the latest version from here.

Structure of IDP02542

2.05 Angstrom Resolution Crystal Structure of D-ribulose-phosphate 3-epimerase from Francisella tularensis.

Edit deposit information
CSGID target
IDP02542 
PDB Id
3INP (NCBI MMDB
Authors
G.Minasov,L.Shuvalova,I.Dubrovska,J.Winsor,P.Scott,W.F.Anderson,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
George Minasov 
Responsible lab
Northwestern University 
Deposition Date
Aug 12, 2009 
Release Date
Aug 25, 2009 

Annotation

Description
D-ribulose-phosphate 3-epimerase from Francisella tularensis catalyses the conversion of D-ribulose 5-phosphate into D-xylulose 5-phosphate and is a part of the pentose phosphate pathway. This metabolic pathway is present in many bacteria and plants, but not animals, making proteins of this pathway a good target for the drug design. Subunits fold in a TIM barrel structure (beta/alpha 8-barrel). A sequence comparison with homologous epimerases outlined the active center and indicated that all members of this family are likely to share the same catalytic mechanism. The substrate could be modeled by putting its phosphate onto the observed sulfate position (between strands beta-7 and beta-8) and its epimerized C3 atom between two carboxylates that participate in an extensive hydrogen bonding system.  
Functional assignment
D-ribulose-phosphate 3-epimerase 

Ligands

Ligand code Name Ligand type
CL
SO4

Structure information

Unit cell parameters

Space Group
F 41 3 2  
Unit Cell

a=224.08Å, b=224.08Å, c=224.08Å
α=90.00, β=90.00, γ=90.00 
Solvent content
71.86  
Matthews coefficient
4.37  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
29.94-2.05Å (2.10-2.05Å)  
Rall(%)
15.9 
Rwork(%)
15.8 (16.1) 
Rfree(%)
17.7 (19.3) 
Num. observed reflections
30664 (2221) 
Num. Rfree reflections
1533 (111) 
Completeness(%)
100.0 (100.0) 

Model parameters

Num Atoms
1728  
Num Waters
165  
Num Hetatoms
185  
Model mean isotropic B factor
13.420Å2  
RMSD bond length
0.012Å  
RMSD bond angle
1.396°  
Filename uploaded
rcsb054638.pdb (uploaded on Aug 18, 2009 2:27 PM)  
Inserted
Aug 18, 2009