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Structure of IDP02733

Glucose-6-phosphate isomerase from Francisella tularensis complexed with fructose-6-phosphate.

Edit deposit information
CSGID target
IDP02733 
PDB Id
3M5P (NCBI MMDB
Authors
J.Osipiuk,N.Maltseva,J.Hasseman,W.F.Anderson,A.Joachimiak 
Responsible person
Jerzy Osipiuk 
Responsible lab
Argonne National Laboratory 
Deposition Date
Mar 12, 2010 
Release Date
Mar 23, 2010 

Annotation

Description
Glucose-6-phosphate isomerase (also known as phosphoglucose isomerase (PGI)) is an enzyme that catalyzes the reversible aldose-ketose isomerization of glucose 6-phosphate (G6P) to fructose 6-phosphate (F6P). The reaction has direct roles in glycolysis and gluconeogenesis and, indirectly, other branches of carbohydrate metabolism such as the pentose phosphate pathway. PGI belongs to the aldose-ketose isomerase family of enzymes, all of which transfer a hydrogen atom between C1 and C2 of their respective substrates. PGIs use sugar substrates that exist in solution overwhelmingly in the hemiacetal or hemiketal ring form. PGIs also have a number of ‘moonlighting’ functions: it acts as an autocrine motility factor (AMF) and neuroleukin agent (NLK), a serine proteinase inhibitor and a differentiation and maturation mediator (DMM). It was found that GPI plays an essential role in the development of arthritis in mammals and may play roles in both cancer and autoimmunity.  
Functional assignment
isomerase 

Ligands

Ligand code Name Ligand type
NA Sodium ion crystallization
PO4 PHOSPHATE ION crystallization
MSE modified residue
F6P fructose-6-phosphate biological
175 3,5-dihydro-5-methylidene-4h-imidazol-4-on

Structure information

Unit cell parameters

Space Group
P 32 2 1  
Unit Cell

a=114.27Å, b=114.27Å, c=83.72Å
α=90.00, β=90.00, γ=120.00 
Solvent content
51.72  
Matthews coefficient
2.55  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
32.10-1.65Å (1.69-1.65Å)  
Rall(%)
13.2 
Rwork(%)
13.1 (28.0) 
Rfree(%)
16.0 (31.5) 
Num. observed reflections
75874 (5453) 
Num. Rfree reflections
3793 (290) 
Completeness(%)
99.7 (98.4) 

Model parameters

Num Atoms
5169  
Num Waters
503  
Num Hetatoms
0  
Model mean isotropic B factor
13.460Å2  
RMSD bond length
0.017Å  
RMSD bond angle
1.453°  
Filename uploaded
idp02733_f6p.pdb (uploaded on Mar 12, 2010 5:36 PM)  
Inserted
Mar 12, 2010