Annotation

Description

Lactamase breaks down a beta-lactam by opening the four-atom ring. Beta-lactam is a common element of a group of antibiotics so-called beta-lactam antibiotics such as penicillins, cephamycins, and carbapennems. Beta-lactamses, produced by some bacteria, are responsible for the resistance to these antibiotics. The beta-lactamase from Y. pestis is a monomer, the overall structure is similar to those of other class C beta-lactamse and all catalytic residues are also in the similar locations in the conserved active site. 

Functional assignment

 

Structure information

Unit cell parameters

Space Group

P 1 21 1  

Unit Cell

a=43.07Å, b=75.80Å, c=59.08Å
α=90.00, β=103.60, γ=90.00 

Solvent content

49.28  

Matthews coefficient

2.42  

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

30.58-1.91Å (1.98-1.91Å)  

R_all(%)

16.3 

R_work(%)

16.1 (23.4) 

R_free(%)

19.8 (27.4) 

Num. observed reflections

30018 (2724) 

Num. R_free reflections

1518 (145) 

Completeness(%)

99.1 (95.0) 

Model parameters

Num Atoms

2692  

Num Waters

305  

Num Hetatoms

305  

Model mean isotropic B factor

20.240Ų  

RMSD bond length

0.013Å  

RMSD bond angle

1.285°  

RMSD dihedral angle

13.996°

 

Filename uploaded

dep.pdb (uploaded on Oct 05, 2010 6:33 AM)  

Inserted

Oct 05, 2010