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Structure of IDP90604

Methionyl-tRNA formyltransferase from Vibrio cholerae.

Edit deposit information
CSGID target
IDP90604 
PDB Id
3Q0I (NCBI MMDB
Authors
J.Osipiuk,M.Gu,L.Papazisi,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Jerzy Osipiuk 
Responsible lab
Argonne National Laboratory 
Deposition Date
Dec 15, 2010 
Release Date
Dec 29, 2010 

Annotation

Description
Methionyl-tRNA formyltransferase transfers formyl group from 10-formyltetrahydrofolate to the aminoacyl moiety of methionyl-tRNA(fMet) which results in two products, tetrahydrofolate and N-formylmethionyl-tRNAfMet. The formylation is the key step in the channeling of the initiator tRNA towards the ribosomal P-site and initiation of translation in eubacteria. The inactivation of the formylase gene on the Escherichia coli chromosome severely impairs cell growth. 
Functional assignment
Transferase 

Ligands

Ligand code Name Ligand type
SO4 crystallization
MSE modified residue
175 3,5-dihydro-5-methylidene-4h-imidazol-4-on

Structure information

Unit cell parameters

Space Group
P 41 21 2  
Unit Cell

a=66.69Å, b=66.69Å, c=183.78Å
α=90.00, β=90.00, γ=90.00 
Solvent content
58.06  
Matthews coefficient
2.93  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
33.40-1.89Å (1.94-1.89Å)  
Rall(%)
17.7 
Rwork(%)
17.6 (28.3) 
Rfree(%)
20.3 (34.1) 
Num. observed reflections
34009 (2435) 
Num. Rfree reflections
1734 (114) 
Completeness(%)
99.5 (98.6) 

Model parameters

Num Atoms
2781  
Num Waters
306  
Num Hetatoms
0  
Model mean isotropic B factor
18.020Å2  
RMSD bond length
0.019Å  
RMSD bond angle
1.710°  
Filename uploaded
x14_refmac2.pdb (uploaded on Dec 15, 2010 7:15 PM)  
Inserted
Dec 15, 2010