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Structure of IDP04610

Crystal Structure of Methyionyl-tRNA Formyltransferase from Bacillus anthracis

Edit deposit information
CSGID target
IDP04610 
PDB Id
3RFO (NCBI MMDB
Authors
Y.Kim,M.Makowska-Grzyska,K.Kwon,W.F.Anderson,A.Joachimiak,Csgid,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Youngchang Kim 
Responsible lab
Argonne National Laboratory 
Deposition Date
Apr 06, 2011 
Release Date
Apr 20, 2011 

Annotation

Description
Methionyl-tRNA formyltransferase modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) at the beginning of translation initiation essential for normal bacterial growth. The structure contains the bigger N-terminal Rossman-fold domain and the C-terminal small beta-barrel domain resembling an OB-fold, positively charged in one side, which presumably interacts with a corresponding tRNA.  
Functional assignment
transferase 

Ligands

Ligand code Name Ligand type
SO4 crystallization
PG5 crystallization
MSE modified residue
SO4 crystallization
GOL crystallization

Structure information

Unit cell parameters

Space Group
P 1 21 1  
Unit Cell

a=62.86Å, b=191.50Å, c=89.95Å
α=90.00, β=89.95, γ=90.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
36.59-2.38Å (2.42-2.40Å)  
Rall(%)
18.3 
Rwork(%)
18.1 (28.3) 
Rfree(%)
21.0 (31.5) 
Num. observed reflections
87076 (3309) 
Num. Rfree reflections
4379 (154) 
Completeness(%)
97.5 (75.0) 

Model parameters

Num Atoms
9855  
Num Waters
504  
Num Hetatoms
558  
Model mean isotropic B factor
58.150Å2  
RMSD bond length
0.004Å  
RMSD bond angle
0.900°  
RMSD dihedral angle
14.03°
 
Filename uploaded
dep.pdb (uploaded on Jan 11, 2013 4:34 PM)  
Inserted
Apr 06, 2011