Annotation

Description

Beta-lactam is a common element of a group of antibiotics so-called beta-lactam antibiotics such as penicillins, cephamycins, and carbapennems. Beta-lactamses, produced by some bacteria, are responsible for the resistance to these antibiotics. The beta-lactamase from Y. pestis is a monomer, the overall structure is similar to those of other class C beta-lactamse and all catalytic residues are also in the similar locations in the conserved active site. Lactamase breaks down a beta-lactam by opening the four-atom ring. The protein was co-crystallized in the presence of Ala-Ala, however, citrate molecule which was not included in the crystallization, was found in the active site. 

Functional assignment

hydrolase 

Structure information

Unit cell parameters

Space Group

P 1 21 1  

Unit Cell

a=43.16Å, b=75.48Å, c=59.07Å
α=90.00, β=103.93, γ=90.00 

Solvent content

 

Matthews coefficient

 

Refinement

Data for the highest resolution shell is in parentheses.

Resolution range

30.51-1.50Å (1.53-1.50Å)  

R_all(%)

15.7 

R_work(%)

15.6 (26.8) 

R_free(%)

17.7 (29.7) 

Num. observed reflections

59809 (2156) 

Num. R_free reflections

3014 (119) 

Completeness(%)

96.8 (74.0) 

Model parameters

Num Atoms

2794  

Num Waters

537  

Num Hetatoms

568  

Model mean isotropic B factor

17.630Ų  

RMSD bond length

0.010Å  

RMSD bond angle

1.330°  

RMSD dihedral angle

14.043°

 

Filename uploaded

dep.pdb (uploaded on May 10, 2011 5:50 PM)  

Inserted

May 10, 2011