Structure of IDP95888

SrtA sortase from Actinomyces oris

Edit deposit information
CSGID target
IDP95888 
PDB Id
5UTT (NCBI MMDB
Authors
J.Osipiuk,X.Ma,H.Ton-That,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Jerzy Osipiuk 
Responsible lab
Argonne National Laboratory 
Deposition Date
Feb 15, 2017 
Release Date
Mar 01, 2017 

Annotation

Description
SrtA from Actinomyces oris is class E sortase. Sortases modify surface proteins by recognizing and cleaving a carboxyl-terminal sorting signal. They are important proteins for pathogenic bacteria The sortases are thought to be good targets for new antibiotics. 
Functional assignment
 

Ligands

Ligand code Name Ligand type
CL CHLORIDE ION crystallization

Structure information

Unit cell parameters

Space Group
P 1 21 1  
Unit Cell

a=62.18Å, b=75.08Å, c=77.65Å
α=90.00, β=89.96, γ=90.00 
Solvent content
 
Matthews coefficient
 

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
37.54-1.70Å (1.74-1.70Å)  
Rall(%)
16.3 
Rwork(%)
16.1 (23.4) 
Rfree(%)
20.5 (28.3) 
Num. observed reflections
74438 (4386) 
Num. Rfree reflections
3647 (273) 
Completeness(%)
94.7 (75.5) 

Model parameters

Num Atoms
5661  
Num Waters
529  
Num Hetatoms
541  
Model mean isotropic B factor
18.370Å2  
RMSD bond length
0.012Å  
RMSD bond angle
1.430°  
Filename uploaded
u6_e3_i_refmac.pdb (uploaded on Feb 16, 2017 4:28 PM)  
Inserted
Feb 16, 2017