Expression

Target
IDP05745  
Expression ID
J14  
Protein Clone
pMCSG28 (May 02, 2013)  
Experiment Date Start
May 13, 2013  
Experiment Date End
May 16, 2013  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-Magic  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
30.0 %  
Inserted
Jun 27, 2013