Expression

Target
IDP00010  
Expression ID
J3  
Protein Clone
pMCSG19c (Apr 21, 2008)  
Experiment Date Start
Apr 25, 2008  
Experiment Date End
Apr 26, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol
CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)-pLysS  
Media Type
2xYT  
Media Volume
0.15 mL  
Growth Temperature
37 °C  
Induction Temperature
25 °C  
Induction Reagent
1mM IPTG  
Experiment Type
assay  
Expression Level
5.0 %  
Solubility Level
100.0 %  
Inserted
Oct 21, 2008