Expression

Target

IDP90121  

Expression ID

W2  

Protein Clone

pMCSG7 (Jan 28, 2009)  

Experiment Date Start

Mar 12, 2009  

Experiment Date End

Mar 14, 2009  

Person

Chris Nelson  

Lab

Washington University  

Protocol

WU_native_soluble_expression_1  

Status

success  

Expression Organism

E.coli  

Expression Strain

BL21(DE3)  

Media Type

AthenaES_SeMET  

Media Volume

8000 mL  

Growth Temperature

37 °C  

Induction Temperature

18 °C  

Induction Reagent

0.5 mM IPTG  

Experiment Type

production  

Expression Level

100.0 %  

Solubility Level

50.0 %  

Inserted

Mar 18, 2009