Clone

Target

IDP01407  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

391  

Reference Sequence Used

MKKSILKSVL ASSITLSITL SSTAFASPIV TPDAPQIAAK GYVLMDYHSG KVLAEKEMDT KLSPASLTKM MTSYVIGQEV KRGNISLNDD VVISKNAWAK NFPDSSKMFV EVGTTVKVSD LNRGIIIQSG NDACVAMAEH VAGTEDAFVD LMNAWASSLG MKNSHFTNSH GLDDPNLYST PYDLALLGQA LIRDVPEEYA IYSEQKFTYN GITQYNRNGL LWDKSMNVDG IKTGHTSGAG YNLVSSATEG NMRLVAVVMG TDNENARKAE SKKLLSYGFR FFETVAPHKA GETFVNETIW MGDKDTIALG VDKDTYVTLP RGQAKDLTAS FVLEKQLKAP LKKGDIVGTL YYQLAGNDIA QYPLLALEDV QEGSLFSRLW DYLVLLFKSW F  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAAAATCAATCCTCAAATCT  

PCR Backward Primer

TTATCCACTTCCAATGTTAAAACCAGCTTTTGAACAGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008