Clone

Target

IDP00162  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

338  

Reference Sequence Used

MRYKKTLMLS IMITSFNSFA FNDNYSSTST VYATSNEATD SRGSEHLRYP YLECIKIGMS RDYLENCVKV SFPTSQDMFY DAYPSTESDG AKTRTKEDFS ARLLAGDYDS LQKLYIDFYL AQTTFDWEIP TRDQIETLVN YANEGKLSTA LNQEYITGRF LTKENGRYDI VNVGGVPDNT PVKLPAIVSK RGLMGTTSVV NAIPNEIYPH IKVYEGTLSR LKPGGAMIAV LEYDVSELSK HGYTNLWDVQ FKVLVGVPHA ETGVIYDPVY EETVKPYQPS GNLTGKKLYN VSTNDMHNGY KWSNTMFSNS NYKTQILLTK GDGSGVKLYS KAYSENFK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGATATAAAAAAACCTTAATGT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTAAAGTTCTCTGAATATGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008