Clone

Target
IDP00013  
Clone Suffix
2
Experiment Start Date
Apr 21, 2008  
Experiment End Date
Apr 22, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG19c  
Sequence Start
1  
Sequence End
372  
Reference Sequence Used
MKTKQTIAAS TLALAMIAGA NSAHAEVNDA TPQQSTGDRL AEIKQHKQGL DAKLQQHKEN VDQTLNELNK VKENVDTKVN ELHERKQVAD EKINEIKQHK QELDAKLQQD KQIAEDKIAE IKEHKKQVED KVAEVKEHKQ NIDNKVNEIK EHKQTVDEKV NEMKQHKENI DQKVNELKEV KKQVDEKLAE LKKAKQTAED KLAELKENKP NTGNTLEELK KIKSNLDSLS ANLELAKQDV KNKLAALQEA RQDLINKINE IKQSKQTVSD DLSKKKQDLD IKINDFKHTE KKIDDKLAEI HTTKQNIDNK INEVSQSKQT ETKTGTTNAN NNARELPNAG SKQSNNMALG MLSVLGGILL VTRNKIKTLF SK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAACAAAACAAACAATTGCT  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTTGAGAATAACGTTTTAATTT  
Shipped To Lab
ANL  
Shipped Date
May 08, 2008  
Inserted
Sep 15, 2008  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-04-25 2008-04-26 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2008-04-25 2008-04-26 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli M9 minimal 1mM IPTG assay
2008-10-20 2008-10-22 Rory Mulligan Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal 1 mM IPTG production
2008-07-16 2008-07-18 Rory Mulligan Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 1 mM IPTG production