Clone

Target

IDP00009  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

363  

Reference Sequence Used

MGNIHIQTKS KEYDVYVGKE SLSHLTTIVQ NMQPSVSNIM IISDEAVASL HLQTVVDALQ IDQKVFSFVV PSGEKEKSFE NFYAAHTSAL ENKLDRNSLI IALGGGMIGD LAGFVAASFM RGIRFVQVPT TLLAHDSAVG GKVAINHPLG KNMIGAFHQP EAVVYHTPFL QSLPEKEWRS GYAEVIKHAL IGDVKLYHWL KEEVQTLADL RDEKLIHILM KAIPVKANIV AQDETEKGVR AHLNFGHTLG HALEKELGYG NITHGDGVAV GMLFAIFLSE QVYKVNLAYE EMKQWFLNYG YPKMPSDLSV ERLVGLMKQD KKANAGTIHM VLMQEYGVVN VVSIPDETVH IALEAFQKDM VEK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGAAACATACATATTCAAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTTTCAACCATATCCTTTTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008