Clone

Target

IDP00012  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

429  

Reference Sequence Used

MKERTIQPVN NGLNGNITIP GDKSISHRAV MFGAIAEGTT TIKGFLPGAD CLSTISCFKE MGVDIVQNGD EVTVVGKGLE GLQEPKAVLD VGNSGTTIRL MSGILANTPF LSCVQGDTSI AKRPMKRVTN PLKQMGANID GREEGTFTPL TIRGGDLKAI EYTSPVASAQ VKSAILLAGL RAEGVTAVTE PHISRDHTER MLEAFGVKVT REGKTVKLAG GQKLTATDVQ VPGDVSSAAF FLVAGAIIPN SKLVLENVGM NPTRTGIIDV LEKMGATFTV EPINEGASEP AANITIETSS LKGIEIGGDI IPRLIDEIPV IALAATQAEG ITVIKDAHEL KVKETNRIDT VVAELTKLGA RIEATDDRMI IYGKSALKGN TVNSYGDHRI GMMLAIAGCI AEGKTIIEDA EAVGVSYPTF FEELQKLAK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAGGAAAGAACAATACAACC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTAGCCAACTTTTGAAGTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008