Clone

Target

IDP00008  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

258  

Reference Sequence Used

MGKVYIVGAG PGDPDLITVK GLKCIEKADV ILYDRLVNKE LLSYAKPEAD LIYCGKLPNY HTMKQETINT FLIKYAKKGK IVTRLKGGDP FVFGRGGEEA EALAKQGVPF EIVPGISAGI AAPAYAGIPV THRDASASFA IVTGHRKEDA AEEVKWGNLA KGVETLAVYM GVSNLPYICE QLMKHGKDKS TPAAIIERGT TSMQRTVVGT LGTIVDVAKK EQIQNPSMIV IGEVVRFREK IHWFEKQTES TYQVSGVL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MGKVYIVGAG PGDPDLITVK GLKCIEKADV ILYDRLVNKE LLSYAKPEAD LIYCGKLPNY HTMKQETINT FLIKYAKKGK IVTRLKGGDP FVFGRGGEEA EALAKQGVPF EIVPGISAGI AAPAYAGIPV THRDASASFA IVTGHRKEDA AEEVKWGNLA KGVETLAVYM GVSNLPYICE QLMKHGKDKS TPAAIIERGT TSMQRTVVGT LGTIVDVAKK EQIQNPSMIV IGEVVRFREK IHWFEKQTES TYQVSGVL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGAAAAGTATATATCGTTGG  

PCR Backward Primer

TTATCCACTTCCAATGTTATAACACTCCACTTACTTGAT  

Shipped To Lab

UT  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008