Clone

Target

IDP00018  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

269  

Reference Sequence Used

MKLNRAIGVI DSGVGGLTVA KELIRQLPKE RIIYLGDTAR CPYGPRSREE VRQFTWEMTE HLLDLNIKML VIACNTATAV VLEEMQKQLP IPVVGVIHPG SRTALKVTNT YHVGIIGTIG TVKSGAYEEA LKSINNRVMV ESLACPPFVE LVESGNFESE MAYEVVRETL QPLKNTDIDT LILGCTHYPI LGPVIKQVMG DKVQLISSGD ETAREVSTIL YHSKMLNEGE EQSDHLFLTT GKIGLFKEIA SKWFGQPIEN VKHIHLEKE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MKLNRAIGVI DSGVGGLTVA KELIRQLPKE RIIYLGDTAR CPYGPRSREE VRQFTWEMTE HLLDLNIKML VIACNTATAV VLEEMQKQLP IPVVGVIHPG SRTALKVTNT YHVGIIGTIG TVKSGAYEEA LKSINNRVMV ESLACPPFVE LVESGNFESE MAYEVVRETL QPLKNTDIDT LILGCTHYPI LGPVIKQVMG DKVQLISSGD ETAREVSTIL YHSKMLNEGE EQSDHLFLTT GKIGLFKEIA SKWFGQPIEN VKHIHLEKE  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAGTTGAATAGAGCAATCG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCTTTTTCTAAATGAATATGTT  

Shipped To Lab

UT  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008