Clone

Target

IDP00020  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

305  

Reference Sequence Used

MNMQEVYEYL STVLPEGHVK QDEMLKNHTH IKVGGKADVF VAPTNYDEIQ EVIKYANKYN IPVTFLGNGS NVIIKDGGIR GITVSLIHIT GVTVTGTTIV AQCGAAIIDV SRIALDHNLT GLEFACGIPG SVGGALYMNA GAYGGEISFV LTEAVVMTGD GELRTLTKEA FEFGYRKSVF ANNHYIILEA RFELEEGVYE EIKAKMDDLT FKRESKQPLE YPSCGSVFKR PPNNFAGKLI QESGLQGKRI GGVEVSLKHA GFMVNVDNGT AQDYIDLIHF VQKTVEEKFG VKLEREVRII GEDKE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATATGCAAGAGGTTTATGA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCTTTATCTTCTCCAATAATC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008