Clone

Target

IDP00006  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

799  

Reference Sequence Used

MRRKAPLKVL SSLAIAAIIG CTSVMSAPLA YAETPAKEKE NVSTTPIDYN LIQEDRLAEA LKERGTINPA SSKEETKKAV EKYIEKKQGD QANKEILPAD TAKEASDFVK KVKEKKMEEK EKVKKPEKNV SPEQKPEPNK KQLNGQVPTS KAKQAPYKGS VRTDKVLVLL VEFSDYKHNN IDQTPGYMYS NDFSREHYQK MLFGNEPYTL FDGSKVKTFK QYYEEQSGGS YTTDGYVTEW LTVPGKASDY GADGSSGHDN KGPKGARDLV KEALHAAAEK GLDLSQFDQF DRYDTNSDGN QNEPDGVIDH LMVIHAGVGQ EAGGGKLGDD AIWSHRSKLA IDPVAIEGTK SKVDYFGGKV AAHDYTIEPE DGAVGVFAHE FGHDLGLPDE YDTKYTGTGS PVEAWSLMSG GSWTGKIAGT EPTSFSPQNK DFLQKNMGGN WAKILEVDYD KIKRGVGVPT YIDQSVTKSN RPGVVRVNLP GKSVETIKPE FGKHAYYSTR GDDMHTTLET PFFDLTKGTN AKFDYKANYE LEAECDFVEV HAVTEDGTKT LIDRLGEKVV QGDKDTTDGK WIDKSYDLSQ FKGKKVKLQF DYITDPAVTY KGFAMDHVNV TVDGQVVFSD DAEGQSKMNL NGFVVSDGTE KKAHYYYLEW RNYAGSDNGL KAGKGPVYNT GLVVWYADDS FKDNWVGVHP GEGFLGVVDS HPEAFVGNLN GKPTYGNTGM QIADAAFSFD QTPAWSVNSL TRGQFNYSGL QGVTTFDDSK VYSNNQIADA GRKVPKLGLK FQVVGQADDK SAGAVWIKR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGAAGAAAAGCGCCACTT  

PCR Backward Primer

TTATCCACTTCCAATGTTAACGTTTAATCCAAACAGCG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008