Clone

Target

IDP00011  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

392  

Reference Sequence Used

MPKSVLYGRN SLEKLWEQSK KFGKRAFIVT DTIMEDLGYV ERCIQQLNTK GITVITYNKV NAEPTNIHVL EALTICKEGK CDFIIGLGGG SCIDAAKAVA VLYTNGGEVE DYVQKDREID NDPLPLIAIP TTSGTGSEVT SVAVITNKRT DVKMMMKHPS FTPQVAIIDP ILTRSVPPHI TAATGIDALC HAVEAYISKV SQSLTDVLAL SAIESIMKYL RIAYEDGENM EAREAMMIAS LQAGIAFSNA SVTLVHGMSR PVGALFHVPH GISNAILLPT VLEYTKGSAV KRLAEIGRCL NKDLYSYCDE EVADYTLGEI KKLCFDLHIP NLKEYGIGEL EFEKAISKMA SDAIESGSPA NNPRVPSYDE IKQLYRECFH YKYEDSIKTL GD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCGAAGTCTGTATTATATG  

PCR Backward Primer

TTATCCACTTCCAATGTTAATCCCCTAATGTTTTTATTGA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008