Clone

Target

IDP00001  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

867  

Reference Sequence Used

MVVKEKVVNE MQEVKEMIDT LVNNGRKALQ ALESFTQEQI DNIVHEMALA GVDQHMPLAK LAVEETGRGV YEDKCIKNIF ATEYIWHSIK KDKTVGIIHE DPHEEIIEIA EPVGVVAGVT PVTNPTSTTM FKALIAIKTR NPIIFAFHPS AQNCSVAAAK TVYDAAVKAG APKHCIQWIE KPSVEATKQL MNHEGVALVL ATGGAGMVKS AYSTGKPALG VGPGNVPCYI EKSAHIKRAV NDLILSKTFD NGMICASEQA IIVDKEIYDD VKTEMITNDC YFVTEEERRK LEKLVINENT CAVNSDIVGK SAQYIAELVG ITVPKNTKML VAEIQGIGAA YPLSREKLSP VLACVKANSL EEGFTYCEEM LNLGGLGHSA VIHSVNKEVQ KQFGLRMKAC RLIVNAPSSQ GGIGDIYNGF IPSLTLGCGS YGKNSVSQNV TATHLLNIKR LANRKKNMQW FKLPPKIYFE KHATAYLANM PNISRAFIVT DPGMVEHGYV DTVAHYLRKH ANDVKVEVFF EVEPDPSDET VFKGAEMMRS FQPDVIIALG GGSAMDAAKG MWLFYEYPET TFYGIKQKFL DIRKRTCKYP ELGSKAQFVA IPTTSGTGSE VTPFAVITDK KNNIKYPLAD YELTPDVAIV DPQFVMTVPP HVTADTGMDV LTHAIEAYVS VLANDYTDGL ALKAIDLVFK YLPRAFKDGH DEEAREKMHN ASAIAGMAFA NAFLGINHSL AHKIGPEFHI PHGRANAILM PHVIRYNAIK PRKHALFPKY EHFVADERYA HIARMLGLPA SSVAEGVESL DKAIIDLGKS LNINMSIAGQ GVAKDQFEEV VGLLAERAFE DQCTTANPKL PLISELKEIY MKAYKGE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGTAGTCAAAGAGAAAGTTG  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCACCTTTATATGCTTTCA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008