Clone

Target

IDP00941  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

413  

Reference Sequence Used

MFMEFSAGLM PLETALTQML SRITPLTAVE TLPLVNCFGR ILATDIVSPL DVPGFDNSAM DGYAVRVADL SADKPLPVAG KAFAGQPYQG EWPAGTCIRI MTGAPVPAGC EAVVMQEQTE QTDDGVRFTA DVRCGQNIRR RGEDIRQDAV VFPAGTRLTT AELPVLASLG IADAQVVRKV RVALFSTGDE LQLPGQPLEA GQIYDTNRLT IHLMLQQLGC EVINLGIIPD DPGKLRAAFI DADSQADVVI SSGGVSVGEA DYTKTILEEL GEIAFWKLAI KPGKPFAFGK LSNSWFCGLP GNPVSAALTF YQLVQPLLAK LGGNTASAVP PRQRVRTASR LKKTPGRLDF QRGILQRSAN GELEVTTTGH QGSHIFSSFS LGNCFIVLER ERGNVEPGEW VEVEPFNALF GGL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTCATGGAATTTTCCGCC  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGGCCTCCGAACAGAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008