Clone

Target
IDP05745  
Clone Suffix
3
Experiment Start Date
Jan 30, 2012  
Experiment End Date
Jan 30, 2012  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
31  
Sequence End
330  
Reference Sequence Used
ENHKTLDGVE TAEYSESYLQ YLEDVKNGDT AKYNGVIPFP HEMEGTTLRN KGRSSLPSAY KSSVAYNPMD LGLTTPAKNQ GSLNTCWSFS GMSTLEAYLK LKGYGTYDLS EEHLRWWATG GKYGWNLDDM SGSSNVTAIG YLTAWAGPKL EKDIPYNLKS EAQGATKPSN MDTAPTQFNV TDVVRLNKDK ETVKNAIMQY GSVTSGYAHY STYFNKDETA YNCTNKRAPL NHAVAIVGWD DNYSKDNFAS DVKPESNGAW LVKSSWGEFN SMKGFFWISY EDKTLLTDTD NYAMKSVSKP  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGGAAAACCATAAAACTCTAGATGG  
PCR Backward Primer
TTATCCACTTCCAATGTTATGGTTTTGATACTGATTTCAT  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-03-06 2012-03-06 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-02-03 2012-02-06 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay