Clone

Target

IDP05745  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Jan 30, 2012  

Experiment End Date

Jan 30, 2012  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

31  

Sequence End

803  

Reference Sequence Used

ENHKTLDGVE TAEYSESYLQ YLEDVKNGDT AKYNGVIPFP HEMEGTTLRN KGRSSLPSAY KSSVAYNPMD LGLTTPAKNQ GSLNTCWSFS GMSTLEAYLK LKGYGTYDLS EEHLRWWATG GKYGWNLDDM SGSSNVTAIG YLTAWAGPKL EKDIPYNLKS EAQGATKPSN MDTAPTQFNV TDVVRLNKDK ETVKNAIMQY GSVTSGYAHY STYFNKDETA YNCTNKRAPL NHAVAIVGWD DNYSKDNFAS DVKPESNGAW LVKSSWGEFN SMKGFFWISY EDKTLLTDTD NYAMKSVSKP DSDKKMYQLE YAGLSKIMSN KVTAANVFDF SRDSEKLDSV MFETDSVGAK YEVYYAPVVN GVPQNNSMTK LASGTVSYSG YINVPTNSYS LPKGKGAIVV VIDNTANPNR EKSTLAYETN IDAYYLYEAK ANLGESYILQ NNKFEDINTY SEFSPCNFVI KAITKTSSGQ ATSGESLTGA DRYETAVKVS QKGWTSSQNA VLVNGDAIVD ALTATPFTAA IDSPILLTGK DNLDSKTKAE LQRLGTKKVY LIGGENSLSK NVQTQLSNMG ISVERISGSD RYKTSISLAQ KLNSIKSVSQ VAVANGVNGL ADAISVGAAA ADNNMPIILT NEKSELQGAD EFLNSSKITK SYIIGGTATL SSNLESKLSN PTRLAGSNRN ETNAKIIDKF YPSSDLKYAF VVKDGSKSQG DLIDGLAVGA LGAKTDSPVV LVGNKLDESQ KNVLKSKKIE TPIRVGGNGN ESAFNELNTL LGK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGGAAAACCATAAAACTCTAGATGG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTTCCTAAAAGAGTATTTAGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Apr 20, 2012