Clone

Target
IDP05283  
Clone Suffix
2
Experiment Start Date
Dec 05, 2012  
Experiment End Date
Dec 06, 2012  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG28  
Sequence Start
1  
Sequence End
91  
Reference Sequence Used
MNIKSVSSNI VVNNYLKNKS DVKKVQQVQK VDKTDKIELS KFAKEMSSVS NMSDEAARNK KIEEIKSALE NGTYKIKPEE IAKKMIEEMR G  
Reference Sequence Variance
MATCH  
PCR Forward Primer
GTCTCTCCCATGAATATTAAAAGTGTAAGTTCAA  
PCR Backward Primer
GGTTCTCCCCAGCTCCTCGCATCTCCTCTATCA  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-12-13 2012-12-13 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2013-01-23 2013-01-23 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay