Clone

Target

IDP05745  

Clone Suffix

7

Sequence Comments

 

Experiment Start Date

Feb 20, 2013  

Experiment End Date

Feb 22, 2013  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG28  

Sequence Start

31  

Sequence End

330  

Reference Sequence Used

ENHKTLDGVE TAEYSESYLQ YLEDVKNGDT AKYNGVIPFP HEMEGTTLRN KGRSSLPSAY KSSVAYNPMD LGLTTPAKNQ GSLNTCWSFS GMSTLEAYLK LKGYGTYDLS EEHLRWWATG GKYGWNLDDM SGSSNVTAIG YLTAWAGPKL EKDIPYNLKS EAQGATKPSN MDTAPTQFNV TDVVRLNKDK ETVKNAIMQY GSVTSGYAHY STYFNKDETA YNCTNKRAPL NHAVAIVGWD DNYSKDNFAS DVKPESNGAW LVKSSWGEFN SMKGFFWISY EDKTLLTDTD NYAMKSVSKP  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCGAAAACCATAAAACTCTAGATGG  

PCR Backward Primer

GGTTCTCCCCAGCTGGTTTTGATACTGATTTCATTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 21, 2013