Clone

Target

IDP01185  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

443  

Reference Sequence Used

MHVNKKIIYV SHDAHFHGAQ LLSLHTIKAL KENFHYSVAI ISIRTGILIH DFQKYGPVYC LEEDYPTENK VESLIKKLLS QDYTIAICST VISGDIVALL AKHNIKVISL IHELPHLIQQ YSAEGKARKI AESAHKIVFP SQYVYEKFHT ITQLDHQKCH ILPQGLFNHN PYKNNIAKAR NELRKKHNLP LDSKIILGVG FADHRKGIDL FSLIAYSVRK IHKNIHFIWV GKTDVHFLNT LSQRYTAHFT LVEPTPDIGL YNAGADLYLL TSREDPFPNV VLEALDTKVP VIGFKNAGGF GDVVTEQTGA LVDYLNLPMM LEKIYEFIGD EDLRLQKGTF GQELIEKNFN FLHYIYQLLN LLEHNYKKIS VIVPNYNYEK YLSERVKSIL NQTYPLYELI FLDDASTDNS VSIFEKLLSN ENKHHLKIQQ IINDKNSGSV FKQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCATGTTAATAAAAAAATCATTTA  

PCR Backward Primer

TTATCCACTTCCAATGCTATTGTTTAAATACAGAACCAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008