Clone

Target

IDP01189  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Sep 04, 2008  

Experiment End Date

Sep 05, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

528  

Reference Sequence Used

MPMFAWKGKR DKSMKVSVVI PVHNEASTLS KVLAEVKKLE PYEIIIVDNG STDGTKEIAL QHHCRVIYYN HSLGNDVGRA IGAREAKGEI VLFLDGDIVM KSEELYPFIK GIQQGHQIVL NNLTWSVYLK VRPHYTTVGK YMLNRYLNKK EFVVGSLIAI PHAISREVIE KLGWWNLADP ALFQAMAMSR GVDISDMASV DVIYTNKVRP VHTGTSPDSP YPKATSRIMG DHLRALQYIT ETYGKRGGFS EGNRDREFIG KYKPVVLKKE KAKYSAIIPV SEEKTTIRSV IQEVKKAGVD EIIIVANGAD IETIKQVNLE NVIVVEVTKT LGNNVARAIG AMHATAEICL FVDGDYAIPA KKLVPFLRAI EDGSDMALND LQCLLDIFHP ADPISMGKYF VNLVAKRPDL WNNSLTAVPH AIRKKVIEKI GYDSLIIPPL AQMKAILEGF SITTVEFVDV IKTNRVRPEQ HEFVNGRISA FDRIFGDQLE AIAYLLQHTD ERGGFTDGDR DREIIQQLRR EEENTNEY  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCTATGTTCGCTTGGAAG  

PCR Backward Primer

TTATCCACTTCCAATGCTAGTATTCATTTGTATTCTCTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008