Clone

Target

IDP00934  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

262  

Reference Sequence Used

MQAEILLTLK LQQKLFADPR RISLLKHIAL SGSISQGAKD AGISYKSAWD AINDMNQLSE HMLVERATGG KGGGGAVLTR YGQRLIQLYD LLGQIQQKAF DVLSDDDALP LDSLLAAISR FSLQTSARNQ WFGTITARDR DLVQQHVDVL LADGKTRLKV ALTAQSGERL GLEEGKEVLI LLKAPWVGIT RDAAVARAAD NQLSGTISHI ERGAEQCEVL MALPDGQTLC ATIPTSDAAT LKEGDDVIAW FNADRVIIAT LC  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAGGCCGAAATCCTTCT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCACAACGTTGCAATAATC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008