Clone

Target
IDP00881  
Clone Suffix
1 - The clone is available in BEIR but it is out of stock.
Experiment Start Date
Sep 08, 2008  
Experiment End Date
Sep 09, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
67  
Reference Sequence Used
MMTYDRNRNA ITTGSRVMIS GTGHTGIIKA IESEGLDAGQ IRRGKTVIVE GCEGKFAPVE LIRLGMN  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MMTYDRNRNA ITTGSRVMIS GTGHTGIIKA IESEGLDAGQ IRRGKTVIVE GCEGKFAPVE LIRLGMN  
PCR Forward Primer
TACTTCCAATCCAATGCGATGATGACATACGATCGTAACC  
PCR Backward Primer
TTATCCACTTCCAATGTCAGTTCATGCCAAGACGAAT  
Shipped To Lab
UT  
Shipped Date
Oct 09, 2008  
Inserted
Oct 13, 2008  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-10-13 2008-10-14 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2008-09-15 2008-09-16 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli M9 minimal 1mM IPTG assay
2008-12-15 Olena Onopriyenko University of Calgary Expression_protocol_Toronto success E. coli Se-Met none production