Clone

Target

IDP05745  

Clone Suffix

9

Sequence Comments

 

Experiment Start Date

May 02, 2013  

Experiment End Date

May 08, 2013  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG28  

Sequence Start

330  

Sequence End

803  

Reference Sequence Used

MPDSDKKMYQ LEYAGLSKIM SNKVTAANVF DFSRDSEKLD SVMFETDSVG AKYEVYYAPV VNGVPQNNSM TKLASGTVSY SGYINVPTNS YSLPKGKGAI VVVIDNTANP NREKSTLAYE TNIDAYYLYE AKANLGESYI LQNNKFEDIN TYSEFSPCNF VIKAITKTSS GQATSGESLT GADRYETAVK VSQKGWTSSQ NAVLVNGDAI VDALTATPFT AAIDSPILLT GKDNLDSKTK AELQRLGTKK VYLIGGENSL SKNVQTQLSN MGISVERISG SDRYKTSISL AQKLNSIKSV SQVAVANGVN GLADAISVGA AAADNNMPII LTNEKSELQG ADEFLNSSKI TKSYIIGGTA TLSSNLESKL SNPTRLAGSN RNETNAKIID KFYPSSDLKY AFVVKDGSKS QGDLIDGLAV GALGAKTDSP VVLVGNKLDE SQKNVLKSKK IETPIRVGGN GNESAFNELN TLLGK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGCCAGATAGTGATAAAAAAATG  

PCR Backward Primer

GGTTCTCCCCAGCTTTTCCTAAAAGAGTATTTAGTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jun 04, 2013