Clone

Target
IDP06092  
Clone Suffix
1
Experiment Start Date
Aug 29, 2013  
Experiment End Date
Sep 04, 2013  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
failed  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
2902  
Reference Sequence Used
MKQFKKKPKK IKRSHQNQKT ILKRPLWLMP LLIGGFASGV YADGTDILGL SWGEKSQKVC VHRPWYAIWS CDKWEEKTQQ FTGNQLITKT WAGGNAANYY HSQNNQDITA NLKNDNGTYF LSGLYNYTGG EYNGGNLDIE LGSNATFNLG ASSGNSFTSW YPNGHTDVTF SAGTINVNNS VEVGNRVGSG AGTHTGTATL NLNANKVTIN SNISAYKTSQ VNVGNANSVI TINSVSLNGD TCSSLARVGV GANCSTSGPS YSFKGTTNAT NTTFSNSSGS FTFEENATFS GAKLNGGAFT FNKKFNATNN TAFNSGSFTF KGTSSFNGAN FSNASYTFNN QATFQNSSFN GGTFTFNDQT NQSTQHPQIQ NSSFSGSATT LKGFATFEQA FNNSNHQLTI QNASFNNATF NNTGKITIEK DASFNNTSFN TPVDTNNMTI SGGVTLSGKN DLKNGATLDF GSSKITLTQG TTFNLTSLGS EKSVTILNSR GGITYNHLLN HAINSLTNAL KTNESSSKPQ SFAQGLWDMI TYNGVTGQLL NENAATSKPT DSSPSKSSTN STQVYQVGYK IGDTIYKLQE TFSHNSIIIQ ALESGTYTPP PVINGSKFDL SASNYINADM PWYNHKYYIP KSQNFTESGT YYLPSVQIWG SYTNSFKQTF SASNSNLVIG YNATWTDHNV SSSDTVAFGD TSGSALNGHC GPWPYYQCTG TTNGTYSAYH VYITANLRSG NRIGTGGAAN LIFNGVDSIN IANATITQHN AGAYSSSMTF STQNMDNSQN LNGLNSNGKL LVYGTTFTNQ AKDGKFIFNA GQATFENTNF NGGSYQFSGD SLNFSNNNQF NSGSFEIGAK NTIFNNANFN NSTSFNFNNS SATTSFVGDF TNANSNLQIA GNAVFGNSTN GSQNTANFNN TGSVNIAGNA TFDNVVFNSP TNTSVKGKVT LNNITLKNLN APLSFGDGTI VFSAHSVINI GEAITNGNPI TLVSSSKAIE YNDAFSKNLW QLINYQGHGA SSEKLVSSAG NGVYDVVYSF NNQTYNFQEV FSPNSISIRR LGVGMVFDYV DMEKSDRLYY QNALGFMTYM PNSYNNNLGN LNNTIYYYDN SIDFYASGKT LFTKAEFSQT FTGQNSAIVF GAKNIWTSVS DAPQSNVIIR FGDNKGAGSN DASGHCWNLQ CIGFITGHYE AQKIYITGSI ESGNRISSGG GASLNFNGLQ GILLTNATLY NRAAGTQSSS MNFVSNSANI QAQNSYFIDD TAQNKGNPNF SFNALNLDFS NSSFRGYVGQ TQSVFKFNAV NAISFTNSSN LSSGLYQMQA KSVLFDNSNL SVSVGTSSIK ANAINLSQNA SINASNHSTL ELQGDLNLND TSSLNLNQSA INVSNNATIN DYASLIASNG SHLNFNGAVN FNSANITTSL SSSSIVFKGA VSLRGQFNLS NNSSLDFQGS SAITSNTAFN FYDNAFSQSP ITFHQALDIK VPLSLGGNLL NPNNSSVLNL KNSQLVFSDQ GSLNIANIDL LSDLNGNKNR VYNIIQADMN GNWYERINFF GMRINDGIYD AKNQTYSFTN PLNNALKITE SFKNNQLSVT LSQIPGIKNT LYNIGSEIFN YQKVYNNANG VYSYSDDAQG VFYLTSSVKG YYNPNQSYQA SGSNNTTKNN NLTSESSVIS QTYNAQGNPI SALHVYNKGY NFSNIKALGQ MALKLYPEIK KILGNDFSLS SLSNLKGDAL NQLTKLITPS DWKNINELID NANNSVVQNF NNGTLIIGAT KIGQTDTNSA VVFGGLGYQK PCDYTDIVCQ KFRGTYLGQL LESISADLGY IDTTFNAKEI YLTGTLGSGN AWGTGGSASV TFNSQTSLIL NQANIVSSQT DGIFSMLGQE GINKVFNQAG LANILGEVAM QSINKAGGLG NLIVNTLGSD SVIGGYLTPE QKNQTLSQLL GQNNFDNLMN DSGLNTAIKD LIRQKLGFWT GLVGGLAGLG GIDLQNPEKL IGSMSINDLL SKKGLFNQIT GFISANDIGQ VISVMLQDIV KPSDALKNDV AALGKQMIGE FLGQDTLNSL ESLLQNQQIK SVLDKVLAAK GLGSIYEQGL GDLIPNLGKK GIFAPYGLSQ VWQKGDFSFN AQGNVFVQNS TFSNANGGTL SFNAGNSLIF AGNNHIAFTN HSGTLNLLSN QVSNINVTML NASNGLKINA TNNNVSVSQG NLFINASCVQ QSDPTTASAT NPCTTAQNNA SSSNASNNAP IALNNNDESL VVTANGFNFS GNIYANGVVD FSKIKGSANV KNLYLYNNAQ FQANNLTISN QAVLEKNASF VTNNLNIQGA FNNNATQKIE VLQNLVIASN ASLSTGIYGL EVGGALNNLG AIHFNLENSQ TPVNPLIQVG GIINLNTTQT PFMNVSVANG GTYTLLKSSR YIDYNINPNS LQSYLKLYTL ININGNHIEE KNGVLTYLGQ RVLLQDKGLL LSVALPNSNN ASQNNILSLS VLHNQIKMSY GNKVMDFTPP TLQDYIVGIQ GQSALNQIEA VGGNNAIKWL STLMMETKEN PLFAPIYLEN HSLNEILGVT KDLQNTASLI SNPNFRNNAT SLLEMASYTQ QTSRLTKLSD FRAREGESNF SERLLELKNK RFSDPNPSEV FVKYSQLSKH PNNLWIQGVG GASFISGGNG TLYGLNVGYD RLVKSVILGG YVAYGYSGFN GNIMHSLANN VDVGMYARAF LKRNEFTLSA NETYGGNASH INSSNSLLSV LNQRYNYNTW TTSVNGNYGY DFMFKQKSVV LKPQVGLSYH FIGLSGMKGK MQNPAYQQFV MHSNPSNESV LTLNMGLESR KYFGKNSYYF VTARLGRDLL IKAKGDNVVR FVGENTLLYR KGEIFNTFAS VITGGEMHLW RLMYVNAGVG LKMGLQYQDL NITGNVGMRV AF  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAACAATTTAAAAAGAAACCAA  
PCR Backward Primer
TTATCCACTTCCAATGCTAAAACGCCACTCGCATGC