Clone

Target

IDP93938  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jan 13, 2014  

Experiment End Date

Jan 23, 2014  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

848  

Reference Sequence Used

MSKVKLAIIG NGMVGHRFIE DLLDKADKDQ FEITVFCEEP RIAYDRVHLS SYFSHHTAEE LSLVREGFYE KHGVKVLVGE RAITINRKEK VIHSNSGRTL YYDKLIMATG SYPWIPPIKG SEGQDCFVYR TIEDLNAIEA CTRRSKRGAV IGGGLLGLEA AGALKSLGVE THVIEFAPVL MAEQLDPMGG DQLRQKIERM GVRVHTGKNT QEIVHSGQNS RKTMLFADGT QLEVDFIVFS TGIRPQDKLA HQCGLATARR GGIAINDYCQ TSDPDVYAIG ECASWQERTF GLVAPGYKMA QVTADHLLGR ENAFQGADMS AKLKLLGVDV GGIGDAHGRT EGARSYVYLD ESKEIYKRLV VSADNKKLLG AVLVGDTSDY GNLLQLALNN IELPENPDSL ILPAHAGSKP AMGVDSLPDT AQICSCFDVT KGDIIQAIGQ GCHTVAALKS ATKAGTGCGG CIPLVTQVLN AELSKQGIEV NHHLCEHFAY SRQELYHLIR VEQIKTFDAL LEKYGKGYGC EVCKPTVGSL LASCWNEYIL EPQHTPLQDT NDNFLGNIQK DGTYSVIPRS PGGEITPDGL LAIGRIAKQY NLYTKLTGSQ RVGMFGAQKD DLPAIWAQLI EAGFETGHAY AKALRMAKTC VGSTWCRFGV GDSVGFGVAL EHRYKGIRTP HKMKFGVSGC TRECSEAQGK DVGIIATENG WNLYVCGNGG MKPRHADLLA ADLDEETLMR YLDRFMMFYI RTADKLQRTS VWLESLEGGI NYLRAVILDD KLGINDQLEA DIARLRDKVT CEWKVTVENP AAQVRFAHFI NSPLRDPNVQ VVPEREQHRP ARPDERILVR VLELEDNL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGCAAAGTCAAACTTGCC  

PCR Backward Primer

TTATCCACTTCCAATGTCATAAGTTATCCTCCAGTTCC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Mar 14, 2014