Clone

Target

IDP01140  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 08, 2008  

Experiment End Date

Sep 09, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

368  

Reference Sequence Used

MKEPLDLSKY SVRTDLAVEA HQMLQERQEE QQQGIQGVIV KEREEEGIII TKVTIDEVAS ESMGKKPGNY LTLEVQGIRQ QDTELQQKVE RIFAKEFSYF LEEVGVTKEA SCLIVGLGNW NVTPDALGPI VVENVLVTRH LFQLQPESVE EGFRPVSAIR PGVMGITGIE TSDVIYGIIE KTKPDFVIAI DALAARSIER VNSTIQISDT GIHPGSGVGN KRKELSKETL GIPVIAIGVP TVVDAVSITS DTIDFILKHF GREMKEGNKP SRSLLPAGFT FGEKKKLTEE DMPDEKSRNM FLGAVGTLED EEKRKLIYEV LSPLGHNLMV TPKEVDAFIE DMANVIASGL NAALHHQIDQ DNTGAYTH  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAGAACCATTAGATTTAAGT  

PCR Backward Primer

TTATCCACTTCCAATGTCAATGTGTATACGCTCCTGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 13, 2008