Clone

Target
IDP01158  
Clone Suffix
1
Experiment Start Date
Sep 08, 2008  
Experiment End Date
Sep 09, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
failed  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
258  
Reference Sequence Used
MSVLEVKGLT KVYQSKGSVA TTALNDINFK IEEGEFVGIM GPSGSGKTTL LNLLATIDKQ TSGHVLVNGE EINQMRAAKL AEFRRTHLGF IFQDFNLLDT LSIKENIILP LVMAKKSVNE IDAKVLEIAK FLNIEEILNK KVYEVSGGQQ QRAAAARAII HEPTLILADE PTGNLDSKSA KSLMSALQDL HEQKRVTIAM VTHDPVAASY CERILFIRDG EIFSEIHKGR TKQAFFQEIL DVLAMLGGEY HELSPARA  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAGTGTTCTTGAAGTAAAGG  
PCR Backward Primer
TTATCCACTTCCAATGTTAAGCGCGAGCTGGCGAA  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-09-15 2008-09-16 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli M9 minimal 1mM IPTG assay
2008-10-13 2008-10-14 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2010-05-24 2010-05-25 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay