Clone

Target

IDP02031  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jul 03, 2014  

Experiment End Date

Jul 11, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7-HaloV2  

Sequence Start

27  

Sequence End

436  

Reference Sequence Used

TINDMQKRQN EIEQKKSEID KNIDSKNSEL NHLESAEKDA AKELESLMKS LDDTNKKLKE QEDKVSSENE KLKKLQKEME KLRNDIRDRQ KVLDNRARAI QTTGTATSYL DMIFEADDFK ELVDRVTVVS AIVKADQNIM QDQKDDQDKL KVAESTSEKK LENLKVLAVE LEVSKNNMES QKQEKNDLVM ALANKKDLTK SEQTLLASEQ GALTDEEKRL ASNIAGEKAK QEAAIKAAEE KRMQEAAAAS SAKSAAVVKQ PSSSSNEATE TVSSGGGQFI KPASGILTSG FSERTNPVTG KYESHKGQDI AGGGTVTVSA AASGTVVFSG FGASGSGFGG YGYVVKIDHG NGFQTLYGHM RAGSLKVVTG QQVSQGQPIG IMGSTGQSTG QHLHFEIHKN GIPVDPAPYI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGACCATCAATGATATGCAAAAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTAAATATATGGTGCTGGATCG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 30, 2014