Clone

Target
IDP95253  
Clone Suffix
1
Experiment Start Date
Sep 19, 2014  
Experiment End Date
Sep 23, 2014  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
151  
Reference Sequence Used
MLEDSVNNGA SIGFLAPIEK NEVLNYWREV NHKLAQGNSR LWIAIQEGTV VGSVQLSLVS KKNGVHRAEV EKLMVLTSAR KQGIATLLLN ELENFSKEKG LRLLVLDTRE GDVSELLYSK IGFVRVGVIP NFALSSNGNY DGTAIYYKKL V  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MLEDSVNNGA SIGFLAPIEK NEVLNYWREV NHKLAQGNSR LWIAIQEGTV VGSVQLSLVS KKNGVHRAEV EKLMVLTSAR KQGIATLLLN ELENFSKEKG LRLLVLDTRE GDVSELLYSK IGFVRVGVIP NFALSSNGNY DGTAIYYKKL V  
PCR Forward Primer
TACTTCCAATCCAATGCGATGTTAGAAGACTCTGTAAATAAC  
PCR Backward Primer
TTATCCACTTCCAATGTCAGACAAGCTTTTTATAATAAATC  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-09-26 2014-09-26 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2014-10-23 2014-10-23 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay