Clone

Target
IDP95254  
Clone Suffix
1
Sequence Comments
I161N;P283H  
Experiment Start Date
Sep 19, 2014  
Experiment End Date
Sep 23, 2014  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG53  
Sequence Start
1  
Sequence End
489  
Reference Sequence Used
MSQEFAIRNH ILAVEVHADR IVIGTDQPFQ TDWLNNLEKS LAPKKIERVF LNPEQLQRYL REYYQVSRAV NSAQKDVAHD RDNKGVEALL QLGDSQNPDA NDQHIVKLVD WILQFAFEQS ASDIHMESRK DNGKVRFRID GVLHTIYNMP SNTLTAVISR IKILGRLNVA EKRKPQDGRL KTRTPKGQET ELRLSTLPTA FGEKLVMRIF DPDVLVRSFQ QLGFDQSLLQ QWQRITQNSH GIILVTGPTG SGKTTTLYSS LKQLATEQVN VCTIEDPIEM LEPSFNQMQV NHAIELGFAD GVRALMRQDP DIIMIGEIRD QDTANMAIQA ALTGHLVLST LHTNDAPSSL TRLHDLGVQP FLTAATILGV LAQRLVRKLC PNCKQQTHIN EDEWQHLTFD YIMEMPEKVY RAVGCEECRH TGYKGRIGIY EFMPVSLELK HLISSHATLN ELRCQTKKES VELLRIAGAR KVIEGLTTLE EVLRVVPLN  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MSQEFAIRNH ILAVEVHADR IVIGTDQPFQ TDWLNNLEKS LAPKKIERVF LNPEQLQRYL REYYQVSRAV NSAQKDVAHD RDNKGVEALL QLGDSQNPDA NDQHIVKLVD WILQFAFEQS ASDIHMESRK DNGKVRFRID GVLHTIYNMP SNTLTAVISR NKILGRLNVA EKRKPQDGRL KTRTPKGQET ELRLSTLPTA FGEKLVMRIF DPDVLVRSFQ QLGFDQSLLQ QWQRITQNSH GIILVTGPTG SGKTTTLYSS LKQLATEQVN VCTIEDPIEM LEHSFNQMQV NHAIELGFAD GVRALMRQDP DIIMIGEIRD QDTANMAIQA ALTGHLVLST LHTNDAPSSL TRLHDLGVQP FLTAATILGV LAQRLVRKLC PNCKQQTHIN EDEWQHLTFD YIMEMPEKVY RAVGCEECRH TGYKGRIGIY EFMPVSLELK HLISSHATLN ELRCQTKKES VELLRIAGAR KVIEGLTTLE EVLRVVPLN  
PCR Forward Primer
TACTTCCAATCCAATGCGATGTCGCAAGAGTTTGCGATC  
PCR Backward Primer
TTATCCACTTCCAATGTTAATTTAATGGAACAACCCGC