Clone

Target

IDP95256  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 19, 2014  

Experiment End Date

Sep 23, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

379  

Reference Sequence Used

MIIAFAIWGS YTKRSTVQGQ LIPQSGLIQV YTTQSGTILK KNVHEGQTVK KGDILFTIST ASYGEQGSIA DALAKQTQLK EQSIRNEITR MRFIHQDEKR TINNQIGLLN GTLVKVENLI ANQRERVNLA KKNQQRYGNI LHENAISHEE FEARKIDYLD QLAQYESLQR EKVSLEKQLT EQQISLSGLE NRQNNQIEQL ERLLSSNTQE LIEMQSRQHI AIQANSSGVI GTINAEVGQF VDLSKPLLTV LPENTALIAQ LYVPSRAIGF VKEGDQVLLR YQAYPYQKFG HAKAQVLSVA KTALASQDLQ TIGIISPQEQ LNNEPVYLVR AKLDKQMVKA YGNDMPLQVG MTLEGDIMHE QRKLYEWVLE PLFSITGKL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTATCGCTTTTGCAATATG  

PCR Backward Primer

TTATCCACTTCCAATGCTATAGTTTTCCTGTAATACTAAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014