Clone

Target

IDP95257  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Sep 19, 2014  

Experiment End Date

Sep 23, 2014  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG53  

Sequence Start

1  

Sequence End

219  

Reference Sequence Used

MINLWKNGYD MVYGIRNRTT ESWLKRVLTQ AYYRILNLSS PIDIPESAGD FRLLDAKVVE AIKQLPEKNR YMKGLYAWVG FKSIGINFSE QERQHGHSSF NLKSLFNLAM SGLTGFSDLP LRVCIYLGAI LALGAMSYGV WIIIKTLIEG ISIPGWATLA AGMTLLGGIQ LLFIGILGEY IGRIYAEVKN RPKYIISTEH SRTKQEHSIA HQENTFSSF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATTAATCTTTGGAAAAATGGT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGAAACTAGAGAACGTATTTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 09, 2014